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human placenta  (TaKaRa)


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    Structured Review

    TaKaRa human placenta
    Human Placenta, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+placenta+cdna/Human+Placenta+Marathon+-Ready+cDNA/us12605398-939-37-39
    Average 93 stars, based on 227 article reviews
    human placenta - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Tracking induced pluripotent stem cell differentiation with a fluorescent genetically encoded epigenetic probe
    Article Snippet: BpiI (BbsI) and Eco31I (BsaI) restriction endonucleases (Thermo Scientific, Waltham, MA, USA) and T4 DNA ligase (Evrogen) were used for the MoClo cloning procedure. .. MPP8 reader domain DNA (aa 55–117) was amplified from human placenta cDNA (Takara Bio, USA) with specific primers based on available sequence NM_017520.4 and cloned into Level 0. ..

    Article Title: Pseudodiastrophic dysplasia expands the known phenotypic spectrum of defects in proteoglycan biosynthesis
    Article Snippet: .. Briefly, a truncated form of wild-type CANT1, lacking the first 64 amino acids (including the predicted transmembrane domain), was amplified from human placenta cDNA (Clontech, Mountain View, California). ..

    Article Title: RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells
    Article Snippet: .. Briefly, the DNA fragment of rasgrp2 was amplified from human placenta cDNA (K1420-1; Clontech) using the Expand Long Range dNTPack (4829042001; Sigma-Aldrich), pEB Multi-Hyg (050-08121; FUJIFILM Wako Pure Chemical Corporation) was used as a vector, and ViaFect Transfection Reagent (E4981; Promega) was used as the transfection reagent. ..

    Article Title: Genetically encoded epigenetic sensors for visualization of H3K9me3, H3K9ac and H3K4me1 histone modifications in living cells.
    Article Snippet: Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation.. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications.. It involves visualization of epigenetic modifications using genetically encoded fluorescent sensors and further analysis of the obtained intranuclear patterns by multiparametric image analysis.

    Article Title: Pseudodiastrophic dysplasia expands the known phenotypic spectrum of defects in proteoglycan biosynthesis.
    Article Snippet: Wild- type human B3GAT3 expression vector was generated as described previously.19 In brief, a truncated form of wild- type B3GAT3, lacking the first 43 amino acids, was amplified from human placenta cDNA and inserted into a p3XFLAG- CMV8 vector (Sigma, St Louis, Missouri). .. Wild- type human CANT1 expression vector was constructed as previously described, with slight modifications.20 Briefly, a truncated form of wildtype CANT1, lacking the first 64 amino acids (including the predicted transmembrane domain), was amplified from human placenta cDNA (Clontech, Mountain View, California). ..

    Sequencing:

    Article Title: Tracking induced pluripotent stem cell differentiation with a fluorescent genetically encoded epigenetic probe
    Article Snippet: BpiI (BbsI) and Eco31I (BsaI) restriction endonucleases (Thermo Scientific, Waltham, MA, USA) and T4 DNA ligase (Evrogen) were used for the MoClo cloning procedure. .. MPP8 reader domain DNA (aa 55–117) was amplified from human placenta cDNA (Takara Bio, USA) with specific primers based on available sequence NM_017520.4 and cloned into Level 0. ..

    Clone Assay:

    Article Title: Tracking induced pluripotent stem cell differentiation with a fluorescent genetically encoded epigenetic probe
    Article Snippet: BpiI (BbsI) and Eco31I (BsaI) restriction endonucleases (Thermo Scientific, Waltham, MA, USA) and T4 DNA ligase (Evrogen) were used for the MoClo cloning procedure. .. MPP8 reader domain DNA (aa 55–117) was amplified from human placenta cDNA (Takara Bio, USA) with specific primers based on available sequence NM_017520.4 and cloned into Level 0. ..

    Plasmid Preparation:

    Article Title: RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells
    Article Snippet: .. Briefly, the DNA fragment of rasgrp2 was amplified from human placenta cDNA (K1420-1; Clontech) using the Expand Long Range dNTPack (4829042001; Sigma-Aldrich), pEB Multi-Hyg (050-08121; FUJIFILM Wako Pure Chemical Corporation) was used as a vector, and ViaFect Transfection Reagent (E4981; Promega) was used as the transfection reagent. ..

    Transfection:

    Article Title: RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells
    Article Snippet: .. Briefly, the DNA fragment of rasgrp2 was amplified from human placenta cDNA (K1420-1; Clontech) using the Expand Long Range dNTPack (4829042001; Sigma-Aldrich), pEB Multi-Hyg (050-08121; FUJIFILM Wako Pure Chemical Corporation) was used as a vector, and ViaFect Transfection Reagent (E4981; Promega) was used as the transfection reagent. ..

    Synthesized:

    Article Title: Genetically encoded epigenetic sensors for visualization of H3K9me3, H3K9ac and H3K4me1 histone modifications in living cells.
    Article Snippet: Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation.. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications.. It involves visualization of epigenetic modifications using genetically encoded fluorescent sensors and further analysis of the obtained intranuclear patterns by multiparametric image analysis.

    Cloning:

    Article Title: Genetically encoded epigenetic sensors for visualization of H3K9me3, H3K9ac and H3K4me1 histone modifications in living cells.
    Article Snippet: Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation.. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications.. It involves visualization of epigenetic modifications using genetically encoded fluorescent sensors and further analysis of the obtained intranuclear patterns by multiparametric image analysis.

    Article Title: Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
    Article Snippet: .. NO: 4 5′-AAGACTAGTGGGCCCCATGCTGATTGA-3′ As the template DNA, human genomic DNA, human placenta cDNA and human kidney cDNA purchased from Clontech were used. (2) Cloning of hGT Gene cDNA Using two combinations of (i) template of human genomic DNA with primers of hGT-5Eco and hGT-7Spe and (ii) template of human placenta cDNA with primers of hGT-2Sal and hGT6Spe, a PCR reaction was performed under the following conditions to obtain fragments of 0.4 kb and 0.8 kb containing an hGT-coding region. (PCR Reaction System) Water was added to 1 μl of template DNA, 5 μl of 10×PCR buffer, 4 μl of dNTPs (200 μM), primer (10 pmol) and 0.5 μl of Tag polymerase (produced by Takara Shuzo) (in the case of Tub polymerase, 0.2 μl) to make 50 μl. (PCR Reaction Condition) First Stage: cycle number: 1, denaturation (94° C.): 5 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Second Stage: cycle number: 30, denaturation (94° C.): 1 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Third Stage: cycle number: 1, denaturation (94° C.): 1 min., annealing (55° C.): 2 min., extension (72° C.): 5 min. Two fragments obtained were combined to construct hGT gene cDNA and subcloned into pBluescriptIISK+(SK). .. The pBluescriptIISK+(SK) was purchased from Stratagene.

    Isolation:

    Article Title: Molecules with extended half-lives, compositions and uses thereof
    Article Snippet: .. Human FcRn was also obtained following isolation from human placenta cDNA (Clontech, Palo Alto. ..

    Polymerase Chain Reaction:

    Article Title: Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
    Article Snippet: .. NO: 4 5′-AAGACTAGTGGGCCCCATGCTGATTGA-3′ As the template DNA, human genomic DNA, human placenta cDNA and human kidney cDNA purchased from Clontech were used. (2) Cloning of hGT Gene cDNA Using two combinations of (i) template of human genomic DNA with primers of hGT-5Eco and hGT-7Spe and (ii) template of human placenta cDNA with primers of hGT-2Sal and hGT6Spe, a PCR reaction was performed under the following conditions to obtain fragments of 0.4 kb and 0.8 kb containing an hGT-coding region. (PCR Reaction System) Water was added to 1 μl of template DNA, 5 μl of 10×PCR buffer, 4 μl of dNTPs (200 μM), primer (10 pmol) and 0.5 μl of Tag polymerase (produced by Takara Shuzo) (in the case of Tub polymerase, 0.2 μl) to make 50 μl. (PCR Reaction Condition) First Stage: cycle number: 1, denaturation (94° C.): 5 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Second Stage: cycle number: 30, denaturation (94° C.): 1 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Third Stage: cycle number: 1, denaturation (94° C.): 1 min., annealing (55° C.): 2 min., extension (72° C.): 5 min. Two fragments obtained were combined to construct hGT gene cDNA and subcloned into pBluescriptIISK+(SK). .. The pBluescriptIISK+(SK) was purchased from Stratagene.

    Produced:

    Article Title: Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
    Article Snippet: .. NO: 4 5′-AAGACTAGTGGGCCCCATGCTGATTGA-3′ As the template DNA, human genomic DNA, human placenta cDNA and human kidney cDNA purchased from Clontech were used. (2) Cloning of hGT Gene cDNA Using two combinations of (i) template of human genomic DNA with primers of hGT-5Eco and hGT-7Spe and (ii) template of human placenta cDNA with primers of hGT-2Sal and hGT6Spe, a PCR reaction was performed under the following conditions to obtain fragments of 0.4 kb and 0.8 kb containing an hGT-coding region. (PCR Reaction System) Water was added to 1 μl of template DNA, 5 μl of 10×PCR buffer, 4 μl of dNTPs (200 μM), primer (10 pmol) and 0.5 μl of Tag polymerase (produced by Takara Shuzo) (in the case of Tub polymerase, 0.2 μl) to make 50 μl. (PCR Reaction Condition) First Stage: cycle number: 1, denaturation (94° C.): 5 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Second Stage: cycle number: 30, denaturation (94° C.): 1 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Third Stage: cycle number: 1, denaturation (94° C.): 1 min., annealing (55° C.): 2 min., extension (72° C.): 5 min. Two fragments obtained were combined to construct hGT gene cDNA and subcloned into pBluescriptIISK+(SK). .. The pBluescriptIISK+(SK) was purchased from Stratagene.

    Construct:

    Article Title: Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
    Article Snippet: .. NO: 4 5′-AAGACTAGTGGGCCCCATGCTGATTGA-3′ As the template DNA, human genomic DNA, human placenta cDNA and human kidney cDNA purchased from Clontech were used. (2) Cloning of hGT Gene cDNA Using two combinations of (i) template of human genomic DNA with primers of hGT-5Eco and hGT-7Spe and (ii) template of human placenta cDNA with primers of hGT-2Sal and hGT6Spe, a PCR reaction was performed under the following conditions to obtain fragments of 0.4 kb and 0.8 kb containing an hGT-coding region. (PCR Reaction System) Water was added to 1 μl of template DNA, 5 μl of 10×PCR buffer, 4 μl of dNTPs (200 μM), primer (10 pmol) and 0.5 μl of Tag polymerase (produced by Takara Shuzo) (in the case of Tub polymerase, 0.2 μl) to make 50 μl. (PCR Reaction Condition) First Stage: cycle number: 1, denaturation (94° C.): 5 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Second Stage: cycle number: 30, denaturation (94° C.): 1 min., annealing (55° C.): 1 min., extension (72° C.): 2 min. Third Stage: cycle number: 1, denaturation (94° C.): 1 min., annealing (55° C.): 2 min., extension (72° C.): 5 min. Two fragments obtained were combined to construct hGT gene cDNA and subcloned into pBluescriptIISK+(SK). .. The pBluescriptIISK+(SK) was purchased from Stratagene.

    Article Title: Pseudodiastrophic dysplasia expands the known phenotypic spectrum of defects in proteoglycan biosynthesis.
    Article Snippet: Wild- type human B3GAT3 expression vector was generated as described previously.19 In brief, a truncated form of wild- type B3GAT3, lacking the first 43 amino acids, was amplified from human placenta cDNA and inserted into a p3XFLAG- CMV8 vector (Sigma, St Louis, Missouri). .. Wild- type human CANT1 expression vector was constructed as previously described, with slight modifications.20 Briefly, a truncated form of wildtype CANT1, lacking the first 64 amino acids (including the predicted transmembrane domain), was amplified from human placenta cDNA (Clontech, Mountain View, California). ..

    Expressing:

    Article Title: Pseudodiastrophic dysplasia expands the known phenotypic spectrum of defects in proteoglycan biosynthesis.
    Article Snippet: Wild- type human B3GAT3 expression vector was generated as described previously.19 In brief, a truncated form of wild- type B3GAT3, lacking the first 43 amino acids, was amplified from human placenta cDNA and inserted into a p3XFLAG- CMV8 vector (Sigma, St Louis, Missouri). .. Wild- type human CANT1 expression vector was constructed as previously described, with slight modifications.20 Briefly, a truncated form of wildtype CANT1, lacking the first 64 amino acids (including the predicted transmembrane domain), was amplified from human placenta cDNA (Clontech, Mountain View, California). ..



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